Fractionation of Unlabelled or Tritiated Hp by HIP Peptide-1 Affinity Chromatography. 1 mg of unlabelled Hp (A) or 40 ng of tritiated Hp (B) was added to a HIP peptide-1 affinity matrix, allowed to bind for 10 min and washed with 10 column volumes of PBS before elution of bound materials with high NaCl as indicated. The X-axis corresponds to fraction number, and the Y-axis corresponds to total μg (A) or DPM (B).
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